Anti-Mouse IgG f(ab) ATTO594 (H+L)

Product name

Anti-Mouse IgG f(ab) ATTO594

Target species

Mouse

Description

ATTO-labeled secondary antibody f(ab) fragments belong to the new generation of fluorescent antibodies characterized by their exceptional fluorescence intensity and superior photostability. Anti-Mouse f(ab) ATTO594 is specifically suited for high and super resolution microscopy, due to the high photostability and strong fluorescence quantum yield.  

Conjugates of f(ab) fragments are preferable to whole antibody conjugates:

- for cellular structures which are sterically difficult to access;

- prevention of interactions with Fc receptor-bearing membranes due to the absence of the Fc region in  f(ab) fragments;

Specification

Goat Anti-Mouse IgG f(ab) ATTO594 (H+L), Immunofluorescence: 1:300-1:500, Degree of labeling (DOL) : >1-3, Unconjugated dye ≤5% of total fluorescence. 

Optical Properties

λex 594nm, λem 627nm

Form

Lyophilized

Nominal concentration

2 mg/ml

Content

0.25mg Anti-Mouse IgG f(ab) ATTO594
1.0 ml Glycerol buffer


Buffer:
50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4.

Clonality

Polyclonal

Isotype

IgG

Purification notes

Column affinity purified, IEX chromatography after papain cleavage.

Storage instructions

Store as glycerol stock at -20°C. Avoid repeated freeze / thaw cycles.

Shipping conditions 

Shipped at ambient temperature.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. 

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